Work

Monosaccharide profiling and osmoprotective effects of camel milk: Implications for protein stability and therapeutic use

Year: 2025

Type: article

Abstract: This study analyzed monosaccharides in cow and camel milk using GC-MS/MS with full scan mode after freeze-drying, N,O-Bis(trimethylsilyl)trifluoroacetamide derivatization, and solid-phase microextraction extraction. Camel milk exhibited a distinct monosaccharide profile, notably enriched in myo-inositol, a known osmolyte. To evaluate its functional role, BSA and α-amylase were used as model proteins. Fluorescence spectroscopy and enzymatic assays showed that both camel milk and myo-inositol stabilized BSA under heat (37, 50, and 70°C) and salt stress (1 M NaCl), whereas cow milk led to structural changes. Myo-inositol at ∼5 mg/dL provided significant protection, matching the effect of camel milk. Camel milk also enhanced amylase activity at 37°C and preserved its function at 70°C, unlike cow milk. These findings suggest that camel milk and its osmolyte content have potential applications in stabilizing proteins under stress, with implications for food science, biotechnology, and pharmaceutical formulations.

Source: Journal of Dairy Science

DOI: 10.3168/jds.2025-27068

Author: Hassan M. Hassan

Institution: United Arab Emirates University

Language: English

Topic: Animal Diversity and Health Studies

Subfield: Food Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: gold

Estimated APC paid: $3,500