Structural flexibility of the nicotinamide group of NADH in butanol dehydrogenase YqdH from Fusobacterium nucleatum
Year: 2025
Type: article
Abstract: Butanol dehydrogenases (BDHs) are NAD(P)H-dependent oxidoreductases that catalyze the reversible conversion of butanol to butyraldehyde. These enzymes play essential roles in microbial butanol fermentation and show significant potential for biofuel synthesis and bioremediation. The crystal structures of BDHs from Fusobacterium nucleatum and Thermotoga maritima have clarified cofactor recognition and proposed reaction mechanisms. However, their distinct cofactor-binding modes and conformational differences in the substrate-binding cleft remain poorly characterized. In this study, we report the crystal structure of Fusobacterium nucleatum butanol dehydrogenase YqdH (FnYqdH) in a partially NADH-bound state. Electron density map analysis showed stable binding of the adenosine and diphosphate groups of NADH to the nucleotide-binding domain of FnYqdH. Conversely, the nicotinamide group was not observed, indicating that it was in an unbound state. Structural comparisons of FnYqdH complexed with either partial ADP or NADH revealed that the adenosine group is stabilized by hydrogen bonds with Thr143, Thr187, and Val184. Nicotinamide group binding induces positional and conformational changes in the diphosphate group of NADH. A comparative analysis of FnYqdH and TmBDH proteins revealed distinct conformational differences between their nucleotide-binding and catalytic domains, including variations in their substrate-binding metal ion sites. In particular, amino acid sequence and structural analyses of the BDH family revealed significant variability in the residues responsible for metal ion binding. Based on the observed flexibility of the nicotinamide group of NADH and the open conformation of FnYqdH, a potential reaction mechanism of FnYqdH is proposed. These findings offer valuable insights into the cofactor and substrate recognition within the BDH protein family.
Source: PLoS ONE
Authors: Xue Bai, Ki Hyun Nam, Yongbin Xu
Language: English
Subfield: Materials Chemistry
Field: Materials Science
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $2,382
Funders: Salt Science Research Foundation, National Research Foundation, Pohang University of Science and Technology, Korea Institute of Science and Technology, Korea Institute of Science and Technology Information, National Research Foundation of Korea, Natural Science Foundation of Liaoning Province, Ministry of Science and ICT, South Korea, Great Southern Development Commission, Government of Western Australia
OpenAlex ID: W4417498699