Year: 2025
Type: article
Abstract: Automated pipetting is a valuable tool for the preparation of high‐throughput experimentation screens; however, automation in postreaction processing (work‐up) steps is often overlooked. Opentrons has created an affordable commercial pipetting robot that unlocks automated liquid dispensing and transferring for even the early‐career researcher. This article demonstrates the utility of this affordable automated pipetting solution for postreaction processing through four case studies, selectively sampling biphasic mixtures when applicable. Work‐up procedures appropriate for high‐throughput screening of academic and industrially relevant reactions, including Suzuki–Miyaura coupling, Buchwald–Hartwig coupling and Miyaura borylation, are described. An additional protocol is designed for determining the appropriate aqueous wash to reduce protodeboronation for both an aryl boronic acid and aryl boronic pinacol ester.
Source: Chemistry - Methods
Authors: Gregory Gaube, Gilian T. Thomas
Institutions: Ruhr University Bochum, University of Leeds
Language: English
Subfield: Organic Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $2,750
Funder: University of Leeds
OpenAlex ID: W4417502180