Work

From lab to industry: Methods for biomass quantification in basidiomycetous fungi

Year: 2025

Type: article

Abstract: Basidiomycota are vital producers of high-value bioactive compounds, enzymes, and natural pigments, making accurate biomass quantification essential for optimizing bioprocesses. This review critically assesses methodologies for measuring biomass concentration in Basidiomycota, including metabolic assays, quantitative real-time PCR (qPCR), ergosterol assays, chitin determination, dissolved oxygen sensors, off-gas analysis, dielectric spectroscopy, flow cytometry, advanced imaging and microscopy, and vibrational spectroscopy. Each technique is systematically evaluated for its scale of application, accuracy, operational mode, and cost-effectiveness, with emphasis on their respective advantages and limitations. By comparing these factors, this review provides guidance in selecting optimal biomass quantification methods to improve efficiency and reliability across laboratory and industrial settings.

Source: Bioresource Technology Reports

DOI: 10.1016/j.biteb.2025.102459

Authors: Saikrishnan Kandaswamy, Pia Bergmann, Katrin Ochsenreither, Lukas Neutsch, Martin Gand, Christoph Lindenberger

Institutions: Ostbayerische Technische Hochschule Amberg-Weiden, Justus-Liebig-Universität Gießen, University of Applied Sciences Kaiserslautern, ZHAW Zurich University of Applied Sciences

Language: English

Topic: Fluid Dynamics and Mixing

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

SDG: Zero hunger

Open Access status: hybrid

Estimated APC paid: $3,920