Work

Chemical Constituents of Excoecaria cochinchinensis with Cytotoxic, Antioxidant, Tyrosinase, and α-Glucosidase Inhibitory Activities

Year: 2025

Type: article

Abstract: Eleven known compounds, including four triterpenoids ( 1 – 4 ), four sterols ( 5 – 8 ), two phenolic compounds ( 9 – 10 ), and a dipeptide ( 11 ), were isolated from Excoecaria cochinchinensis . Their structures were confirmed through spectroscopic analysis and compared with the existing literature. Eight compounds ( 1 , 2 , 5 – 8 , 10 – 11 ) have been identified in this species for the first time. The cytotoxicity of isolated compounds against MCF-7, A549, and HeLa cancer cell lines, antioxidant, α- glucosidase, and tyrosinase inhibitory activities were evaluated. The results revealed moderate cytotoxic effects against MCF-7 cells ( 2 – 4 ), A549 cells ( 1 – 4 ), and HeLa cells ( 1 – 5 ), while compounds 6 – 8 , 10 , and 11 showed no activity in any of the cell lines tested. Compound 1 exhibited more potent antioxidant activity and tyrosinase inhibitory properties than the positive control. Meanwhile, compound 4 showed a significant inhibitory effect on the α- glucosidase. In silico study, the molecular docking simulation of compound 1 to antioxidant (3RP8) and tyrosinase proteins (2Y9X), and of compound 4 to MCF-7 (1H7K), A549 cells (4ZXT), and α -glucosidase proteins (MAL32) were performed to calculate their binding affinities and to identify the ligand-binding sites.

Source: Revista Brasileira de Farmacognosia

DOI: 10.1007/s43450-025-00731-y