Work

High-Dimensional Protein Analysis Uncovers Distinct Immunologic and Stromal Features in Primary and Metastatic Pancreatic Ductal Adenocarcinoma

Year: 2025

Type: article

Abstract: Pancreatic ductal adenocarcinoma (PDAC) is an aggressive tumor type with poor patient outcomes. Most patients present with metastatic disease, which generally has reduced immune infiltration compared with primary tumors. Further work to elucidate the specific cellular features of metastatic PDAC is needed to guide the development of future immunotherapy strategies. In this study, we investigated the hypothesis that PDAC tumors harbor distinct immunologic and stromal features depending on their anatomic site. Multiplex IHC, spatial analysis, and single-cell mass cytometry (CyTOF) uncovered dominant immune and stromal cell populations in tumors derived from 27 primary and 26 liver metastases. Metastatic liver tumors from patients with PDAC contained reduced T-cell infiltration, fibroblast populations, and collagen accumulation than primary lesions, whereas CD68+ cells, often coexpressing CCR2, were more abundant. Spatial analyses revealed distinct immune cell communities in primary and metastatic PDAC, in which cytokeratin 19 (CK19+) cells clustered differentially with αSMA+, CD3+, and CD68+ cells, depending on the tumor site. When comparing tumor-associated regions, the proportion of peritumoral CK19- cells remained consistent, but their composition varied by disease site. CD8+ T cells were significantly less frequent in metastatic tumors, whereas both CD4+ and CD8+ T cells present in primary tumors expressed more transcription factors associated with suppressive properties, including FoxP3 and RORγt. CyTOF revealed that T cells coexpressed multiple inhibitory checkpoint receptors, most notably LAG3 and PD-1. This report reveals that primary and metastatic tumors from patients with PDAC harbor vastly distinct immunologic and stromal features at the protein level. SIGNIFICANCE: Protein level analysis reveals distinct immunological and stromal features between primary and metastatic pancreatic tumors, offering a rationale for immunotherapies that target macrophages and increase T-cell abundance in metastatic disease.

Source: Cancer Research

DOI: 10.1158/0008-5472.can-25-1697