Work

Molecular Authentication of Commercially Cultivated Coffee (Coffea spp.) in the Philippines using DNA Barcodes

Year: 2020

Type: article

Abstract: Accurate identification of commercially cultivated coffee species is necessary since the cup quality may be attributed to the kind of species used. Hence, DNA barcoding was performed using nuclear ribosomal internal transcribe spacer (ITS) and maturase K (matK). Both markers had 100% amplification and sequencing success rates. Although ITS had lower resolution in Coffea species, it efficiently discriminated Coffea liberica. The matK barcode discriminated all the species. Findings revealed that matK is an efficient barcode over ITS for commercially cultivated Coffea species by generating the highest rate of both universality and discrimination power. DNA barcoding as a method of authentication will benefit the coffee industry and coffee growers for large-scale plantations.

Source: International Journal of Agriculture and Biology

DOI: 10.17957/ijab/15.1660

Authors: Arleen C. Panaligan, Miriam D. Baltazar, Grecebio Jonathan D. Alejandro

Institutions: Cavite State University, University of Santo Tomas

Language: English

Topic: Identification and Quantification in Food

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Zero hunger

Open Access status: bronze

Funders: Commission on Higher Education, National Museum of Natural History, Philippine Council for Agriculture, Aquatic and Natural Resources Research and Development