Work

CePO 4 Nanoneedles as Nanozyme Electrochemical Biosensors for Myoglobin Detection

Year: 2025

Type: article

Abstract: High Resolution Image Download MS PowerPoint Slide In this study, we developed a CePO 4 nanoneedle as a nanozyme-based electrochemical immunosensor for detecting myoglobin (MYO), a key biomarker for the early diagnosis of myocardial infarction. The CePO 4 nanoneedles were synthesized hydrothermally and thoroughly characterized by spectroscopic, structural, and morphological techniques. Their high aspect ratio and redox-active Ce 3 + /Ce 4 + sites enable both efficient biomolecule immobilization and intrinsic electron mediation. The stepwise electrode modification was confirmed by FTIR and SEM analyses, which verified the successful functionalization and uniform surface coverage of the working electrode. Without requiring an external redox probe, the CePO 4 /Myo nanozyme-based immunosensor exhibited a linear response from 100 to 400 ng mL –1, a detection limit of 100 ng mL –1, and a correlation coefficient ( R 2 ) of 0.96. The sensor maintained stable performance for up to 21 days of storage at 4 °C, and matrix analyses confirmed its reliable detection capability in simulated real samples. Overall, this work highlights the intrinsic redox functionality of CePO 4 nanoneedles, which act as nanozymes, enabling electrochemical sensing without the need for conventional redox probes. Their integration into a point-of-care platform demonstrates great potential for simple, portable, and accessible diagnostic tests.

Source: ACS Applied Nano Materials

DOI: 10.1021/acsanm.5c04076