Fabrication of Double‐Network Hydrogel Based on N,O ‐carboxymethyl chitosan, Aldehyde Hyaluronate, and Alginate for 3D Bio‐Ink Applications
Year: 2025
Type: article
Abstract: ABSTRACT This study fabricates and characterizes novel double‐network hydrogels for 3D bio‐ink applications, combining N,O ‐carboxymethyl chitosan (NOCC), aldehyde hyaluronate (AHy), and alginate (Alg). The initial Schiff base‐cross‐linked network (NOCC–AHy–Alg) is enhanced with a second ionic Ca 2+ cross‐linking, optimized via an internal suspension method (CaCO 3 /glucono‐δ‐lactone). Optimal parameters are 0.2 m CaCO 3 and 0.1 m glucono‐δ‐lactone with a 1‐h immersion, proving superior to external CaCl 2 immersion. This internal approach yields a more robust network with improved compressive strength, dimensional stability, and lower porosity, requiring sustained Ca 2+ for long‐term stability. The hydrogels also demonstrate excellent self‐recovery and energy dissipation, indicating potential for 3D printing. In vitro studies confirm biocompatibility with L929 and AT‐MSCs. Overall, these double‐network hydrogels show significant promise as advanced bio‐inks for tissue engineering and other biomedical uses.
Source: Macromolecular Bioscience
Authors: Binh Thanh Vu, Tuan‐Ngan Tang, Dai‐Tin Luong, Thao Thi‐Phuong Nguyen, Thao Thi‐Phuong Nguyen, Yen‐Nhi Ha‐Nguyen, Nhu‐Thuy Trinh, Hang Thi‐Thuy Cao, Thi‐Hiep Nguyen, Thi‐Hiep Nguyen
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: bronze
Award: G3101266956
OpenAlex ID: W4417539113