Work

Sensitive Assay for Mycoplasma Detection in Mammalian Cell Culture

Year: 2012

Type: article

Abstract: Mycoplasma contamination in mammalian cell cultures is often overlooked yet is a serious issue which can induce a myriad of cellular changes leading to false interpretation of experimental results. Here, we present a simple and sensitive assay to monitor mycoplasma contamination (mycosensor) based on degradation of the Gaussia luciferase reporter in the conditioned medium of cells. This assay proved to be more sensitive as compared to a commercially available bioluminescent assay in detecting mycoplasma contamination in seven different cell lines. The Gaussia luciferase mycosensor assay provides an easy tool to monitor mammalian cell contaminants in a high-throughput fashion.

Source: Analytical Chemistry

DOI: 10.1021/ac2033112

Authors: M. Hannah Degeling, Casey A. Maguire, M. Sarah S. Bovenberg, Bakhos A. Tannous

Institutions: Harvard University, Leiden University Medical Center

Language: English

Topic: Microbial infections and disease research

Subfield: Microbiology

Field: Immunology and Microbiology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Cancer Institute, National Institute of Neurological Disorders and Stroke

Awards: G2877435986, Glioblastoma stem cells therapy, Imaging and therapy of tumors using biotinylated cell surface docking sites, Interdepartmental Neuroscience Center, G901775365