The Use of C14O2 Yields from Glucose-1- and -6-C14 for the Evaluation of the Pathways of Glucose Metabolism
Year: 1963
Type: article
Abstract: Katz and Wood (1, 2) examined methods that use differently labeled types of glucose for the evaluation of pathways of glucose metabolism.They indicated that any method must encompass several considerations:(a) the pentose cycle' entails synthesis of fructose 6-phosphate and therefore there may be recycling of the hexose phosphates; (b) in addition to the pentose cycle and Embden-Meyerhof pathways, some of the carbohydrate may be metabolized by nontriose phosphate pathways;' (c) the oxidation of triose phosphate to CO2 usually is incomplete.The relative yield of Cl4 from glucose-l-Cl4 and glucose-6-Cl4 in triose phosphate derivatives such as lactate, fatty acids, or CO% was shown (1, 2) to be a nonlinear function of two or three independent variables with no simple relationship to the separate pathways.However, it was shown that when metabolism by only the pentose cycle and Embden-Meyerhof pathways occurs, the pathways can be evaluated from the ratio of Cl4 in triose-P
Source: Journal of Biological Chemistry
Authors: Joseph Katz, Harland G. Wood
Institution: Case Western Reserve University
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $3,430
OpenAlex ID: W54700548