Work

Serum-Free Recombinant Production of Adenovirus Using a Hollow Fiber Capillary System

Year: 2001

Type: article

Abstract: A novel method for the production of adenoviral vectors on a scale sufficient to support most research applications and early phase clinical trials is presented. This method utilizes serum-free cell culture medium and a hollow fiber cell culture apparatus. Significantly less time and space are required than in conventional methods, and the resulting adenovirus is collected in a much smaller volume, simplifying the purification steps. The protocol described is a reproducible, convenient, biologically safe, and environmentally sound method for the production of adenoviral vectors for laboratory use and has the potential to scale-up the adenovirus production for clinical use.

Source: BioTechniques

DOI: 10.2144/01302pf01

Authors: T.A. Gardner, S.-C. Ko, L. Yang, J.J.S. Cadwell, L.W.K. Chung, C. Kao

Institution: University of Virginia

Language: English

Topic: Virus-based gene therapy research

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,415