3D image deconvolution in light microscopy : theory and practice
Year: 2000
Type: report
Abstract: The objective of this study is to investigate the use of deconvolution for improving the three-dimensional resolution in both confocal and wide-field light microscopy. We start by assessing the theoretical limits of the deconvolution by defining and calculating a characteristic function which describes the imaging-deconvolution process. We continue by suggesting a variant of inverse filtering for deconvolving real noisy data and describe the application of the method to a confocal recording of a biological preparation. The result is comparable with that obtainable by Wiener filtering but we can avoid the problem of finding a best value of the parameter. We also describe a way of determining the optical transfer function of the microscope.
Author: Johan Philip
Language: English
Subfield: Computer Vision and Pattern Recognition
Field: Computer Science
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W562062055