Work

Cytoplasmic lipid bodies of human eosinophils. Subcellular isolation and analysis of arachidonate incorporation.

Year: 1991

Type: article

Abstract: Lipid bodies are non-membrane-bound cytoplasmic inclusions that are prominent in leukocytes engaged in inflammatory responses. As demonstrated by electron microscopic autoradiography, lipid bodies can serve as intracellular sites of 3H-arachidonic acid localization in eosinophils and other cells. To evaluate the role of lipid bodies as stores of esterified arachidonate, subcellular fractionation of lipid-body-rich human eosinophils was used to isolate lipid bodies free of other organelles. In lipid bodies isolated from 3H-arachidonate-labeled eosinophils, 3H-arachidonate was esterified almost totally in glycerolipids, predominantly in classes of phospholipids, including phosphatidyl-inositol and phosphatidylcholine. Lipid bodies, especially in leukocytes participating in inflammation, could represent intracellular sources of esterified arachidonate available for eicosanoid formation.

Source: PubMed Central

Authors: P F Weller, R A Monahan-Earley, H F Dvorak, A M Dvorak

Institution: Harvard University

Language: English

Topic: Lipid metabolism and biosynthesis

Subfield: Biochemistry

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Cancer Institute, National Institute of Allergy and Infectious Diseases

Awards: BASOPHIL/MAST CELL FUNCTION, Cytoplasmic Lipid Bodies of Inflammatory Cells