Work

Quantitative Tracking of Protein Trafficking to the Nucleus Using Cytosolic Protein Delivery by Nanoparticle-Stabilized Nanocapsules

Year: 2015

Type: article

Abstract: We describe a method for quantitative monitoring of subcellular protein trafficking using nanoparticle-stabilized nanocapsules for protein delivery. This method provides rapid delivery of the protein into the cytosol, eliminating complications from protein homeostasis processes found with cellularly expressed proteins. After delivery, nuclear protein trafficking was followed by real time microscopic imaging. Quantitative analyses of the accumulation percentage and the import dynamics of the nuclear protein trafficking, demonstrate the utility of this method for studying intracellular trafficking systems.

Source: Bioconjugate Chemistry

DOI: 10.1021/acs.bioconjchem.5b00141

Authors: Moumita Ray, Rui Tang, Ziwen Jiang, Vincent M. Rotello

Institution: University of Massachusetts Amherst

Language: English

Topic: Nanoparticle-Based Drug Delivery

Subfield: Biomaterials

Field: Materials Science

Domain: Physical Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of General Medical Sciences, National Institute of Biomedical Imaging and Bioengineering

Awards: G1615300055, G2733477506, Intracellular Host-Guest Activation of Nanoparticle Therapeutics, G6099105179