Expression and Purification of Recombinant Saposin B for Coenzyme Q10 Purification
Year: 2012
Type: article
Abstract: Coenzyme Q10 (CoQ10) plays a critical role in energy production in humans. Low levels of CoQ10 have been linked to diseases such as Parkinson's and Huntingtons's disease. CoQ10 levels decrease as we age, and use of statin drugs also lowers CoQ10 levels. Oral supplementation however increases CoQ10 levels. However, the lack of industrial sources of CoQ10 and the difficulty in CoQ10 purification has resulted the need to address these problems. Sporidiobolus johnsonii (S. johnsonii) has been reported as a natural producer of CoQ10. This work is aim to increase the production of CoQ10 in S. johnsonii through genetic engineering. Current purification methods for CoQ10 are difficult and expensive. A protein based purification method may alleviate the current problems associated with typical LC purification. Saposin B (Sap B) has been shown to bind with CoQ10 at selective pH's. We hypothesized that utilizing a Sap B coated support resin that an affinity purification method for CoQ10 could e produced. To this end, the recombinant Sap B was expressed and bound via a HIS-Tag to a sepharose IMAC bead. This work demonstrates that CoQ10 can be bound and released by a Sap B based affinity resin by manipulating the pH.
Author: Vivian Yaci Yu
Institution: Syracuse University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W6186542