Work

Identification of adeno-associated virus contamination in cell and virus stocks by PCR

Year: 2004

Type: article

Abstract: To further understand the biology of adeno-associated virus (AAV) and identify the presence of AAV in laboratory samples, we have developed a sensitive PCR-based assay using degenerate primers based on the sequence of seven diverse AAV isolates. Using these primers, we can detect free virus in viral stocks, cleared cell lysate, as well as in latently infected cells. The method can detect as little as 10 viral copies/microL of sample and can be adapted for high-throughput screening technology. With this method, we have also detected a new AAV isolate from a stock of bovine adenovirus.

Source: BioTechniques

DOI: 10.2144/04364dd01

Authors: Hisako Katano, Sandra Afione, Michael Schmidt, John A. Chiorini

Institution: National Institutes of Health

Language: English

Topic: Virus-based gene therapy research

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,415