Year: 1986
Type: article
Abstract: We reported herein a simple and highly sensitive radioimmunoassay (RIA) for 8-arginine vasopressin (AVP) in human plasma and its application for clinical purpose.ODS Cis column was used for simple extraction of AVP from plasma.It gave high recovery rate (87.1 ± 10.4%, Mean ± SD) in the range of 1-10pglml when authentic AVP was added to O.5ml plasma, and eliminated completely nonspecific substances which interfered with RIA.Specific antiserum was generated against AVP and permited highly sensitive RIA whose standard curve covered AVP range from O.025 to 8pglml.The within and between assay valiability was about 10% each.Using this method, we demonstrated that plasma AVP levels in normal subjects (n==65) ranged from O.30 to 4.20pglml while those in patients with diabetes insipidus (DI) did from O.03 to O.21pglml (n=13) or less than O.03pg/ml (n==3).Thus, this assay method clearly differenciated patients with DI from normal subjects.Plasma AVP levels of normal subjects (n=6) in standing, sitting and supine position after overnight fluid deprivation were 2.41 ± 1.l5, 1.95 ± O.85 and O.97 ± O.48pglml respectively measured 30min after each change of position.Plasma AVP concentration of normal subjects (n==6) after water load (20.mlfkg body weighO were clearly reduced from 1.89 ± 1.00 to O.42 ± O.21 (standing for 60min) and also from O.89 ± O.41 to O.40 ± O.22 pglml (supine for 60min).In conclusion, we developed a simple and highly sensitive AVP assay using plasma extraction and specific RIA.This method is worthwhile for clinical application.
Authors: 兵一郎 桜井, 晃 金井, 馨 野村, 博 出村, 和夫 鎮目, Hyoichiro Sakurai, Akira Kanai, Kaoru Nomura, Hiroshi Demura, KAZUO SHIZUME
Language: Japanese
Subfield: Nephrology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W627231536