Work

Enzymic determination of d (−)-β-hydroxybutyric acid and acetoacetic acid in blood

Year: 1962

Type: article

Abstract: The current methods for the estimation of f,hydroxybutyrate (e.g.Greenberg & Lester, 1944; Thin & Robertson, 1952; Bloom, 1958; Bessman & Anderson, 1957) all involve its oxidation to acetone and the estimation of the latter.These methods are laborious and have several unsatisfactory features.They are unspecific in that other compounds also yield acetone on oxidation and they do not differ- entiate between the optical isomers.Moreover, the conversion of f-hydroxybutyrate into acetone is not quantitative and the yield varies with the con- ditions.The method described in this paper is in principle more specific and more rapid than the older procedures.It is analogous to the enzymic determination of many other metabolites.It depends on the spectrophotometric measurement of

Source: Biochemical Journal

DOI: 10.1042/bj0820090

Authors: DH WILLIAMSON, J MELLANBY, HA KREBS

Institutions: University of Oxford, Medical Research Council

Language: English

Topic: Metabolism and Genetic Disorders

Subfield: Clinical Biochemistry

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: Rockefeller Foundation, National Institutes of Health, U.S. Public Health Service

OpenAlex ID: W62898540