Work

Live Cell Imaging of RhoGTPase Biosensors in Tumor Cells

Year: 2013

Type: book-chapter

Abstract: Tumor cell motility and invasion rely on actin cytoskeleton rearrangements mediated by the activation of RhoGTPase signaling pathways. Invadopodia are membrane-degrading protrusions that mediate extracellular matrix degradation. Here, we provide procedures for imaging RhoGTPase biosensors in tumor cells during the formation of invadopodia and matrix degradation.

Source: Methods in molecular biology

DOI: 10.1007/978-1-62703-538-5_22

Authors: Jose Javier Bravo-Cordero, Yasmin Moshfegh, John Condeelis, Louis Hodgson

Institutions: Albert Einstein College of Medicine, Yeshiva University

Language: English

Topic: Protein Kinase Regulation and GTPase Signaling

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Cancer Institute, National Institute of General Medical Sciences