Work

Alfalfa-Verticillium Albo-atrum Interactions : In Vitro Responses of Alfalfa Callus to Fungal Culture Filtrate and Cell Wall Components

Year: 1992

Type: article

Abstract: Interactions between alfalfa and Verticillium albo-atrum were examined in terms of the following aspects:growth of V. albo-atrum on callus;growth of callus on the medium containing culture filtrate of V. albo-atrum and production of antifungal substances in callus treated with cell wall components (elicitor) from V. albo-atrum. Mycelial growth of V. albo-atrum was likely to be suppressed on the callus from resistant cultivars (Vertus and 5444), accompanied by strong browning. The growth of callus from Vertus (resistant, R) and Kitawakaba (susceptible, S) were vigorous on the medium containing 0.5 or 1% culture filtrate, while those from 5444 (R) and Europe (S) were inhibited. Antifungal substances were detected in all cultivars treated with cell wall components, with concentrations being higher in resistant than in susceptible cultivars. These results suggest that antifungal activity in the callus extracts indicated by the inhibition of germ tube elongation of fungal conidia is the best criterion for the selection of resistant cultivars.

Source: Obihiro University of Agriculture and Veterinary Medicine Institutional Repository

DOI: 10.14941/grass.37.412

Authors: Masanori Koike, Kouhei Nanbu, Tohru Shimada

Institution: Obihiro University of Agriculture and Veterinary Medicine

Language: English

Topic: Plant-Microbe Interactions and Immunity

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: green

OpenAlex ID: W642127084

FWCI:

Cites:

Cited by: 1

Related to: 10