Work

XL-MS: Protein cross-linking coupled with mass spectrometry

Year: 2015

Type: review

Abstract: With the continuing trend to study larger and more complex systems, the application of protein cross-linking coupled with mass spectrometry (XL-MS) provides a varied toolkit perfectly suited to achieve these goals. By freezing the transient interactions through the formation of covalent bonds, XL-MS provides a vital insight into both the structure and organization of proteins in a wide variety of conditions. This review covers some of the established methods that underpin the field alongside the more recent developments that hold promise to further realize its potential in new directions.

Source: Methods

DOI: 10.1016/j.ymeth.2015.06.010

Author: Andrew N. Holding

Institutions: University of Cambridge, Cancer Research UK, Cancer Research UK Cambridge Center

Language: English

Topic: Mass Spectrometry Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: hybrid

Estimated APC paid: $3,158

Funder: Medical Research Council