Identification of antigenic site within hsc 70 by serum autoantibody in patients with cancer-associated retinopathy
Year: 1999
Type: article
Abstract: In our previous studies, both recoverin and heat shock cognate protein 70 (hsc 70) were found as autoantigens recognized by sera from four patients with cancer-associated retinopathy (CAR) . In the present study on the mole-cular mechanism of antibody generation in CAR, we identified the antigenic site within hsc 70 by the patients' sera using deletion mutants of hsc 73. We expressed a series of deletion mutants of hsc 73 proteins and subjected then to western blot analysis. In western blot analysis, CAR patient's serum reacted with wild type hsp 70, but not with the C-terminal truncated mutants. This data demonstrated that the antigenic site was located within the C-terminus region of hsc 73 as identified by CAR patient's serum.
DOI: 10.15114/tr.34.49
Authors: Tadao Maeda, Hiroshi Ohguro, Akiko Maeda, Kei Ogawa, Takashi Nakagawa, Itaru Hirai, Noriyuki Sato
Institution: Sapporo Medical University
Language: English
Topic: Heat shock proteins research
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W655334366