Inter-laboratory evaluation of instrument platforms and experimental workflows for quantitative accuracy and reproducibility assessment
Year: 2015
Type: article
Abstract: The reproducibility of plasma protein quantitation between laboratories and between instrument types was examined in a large-scale international study involving 16 laboratories and 19 LC–MS/MS platforms, using two kits designed to evaluate instrument performance and one kit designed to evaluate the entire bottom-up workflow. There was little effect of instrument type on the quality of the results, demonstrating the robustness of LC/MRM-MS with isotopically labeled standards. Technician skill was a factor, as errors in sample preparation and sub-optimal LC–MS performance were evident. This highlights the importance of proper training and routine quality control before quantitation is done on patient samples.
Source: EuPA Open Proteomics
Authors: Andrew J. Percy, Jessica Tamura-Wells, Juan Pablo Albar, Kerman Aloria, Ardeshir Amirkhani, Gabriel D.T. Araujo, Jesus M. Arizmendi, Francisco J. Blanco, Francesc Canals, Jin-Young Cho, Núria Colomé-Calls, Fernando J. Corrales, Gilberto Domont, Guadalupe Espadas, Patricia Fernandez-Puente, Concha Gil, Paul A. Haynes, Maria Luisa Hernáez, Jin Young Kim, Arthur Kopylov, Miguel Marcilla, Mathew J. McKay, Mehdi Mirzaei, Mark P. Molloy, Leanne B. Ohlund, Young-Ki Paik, Alberto Paradela, Mark Raftery, Eduard Sabidó, Lekha Sleno, Daniel Wilffert, Justina C. Wolters, Jong Shin Yoo, Victor Zgoda, Carol E. Parker, Christoph H. Borchers
Institutions: University of Victoria, Genome British Columbia, Centro Nacional de Biotecnología, Universidad Autónoma de Madrid, University of the Basque Country, Macquarie University, Universidade Federal do Rio de Janeiro, Universidade da Coruña, Instituto de Salud Carlos III, Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine, Centro de Investigación Biomédica en Red, Complexo Hospitalario Universitario A Coruña, Servicio Gallego de Salud, Instituto de Investigación Biomédica de A Coruña, Vall d'Hebron Institut de Recerca, Vall d'Hebron Hospital Universitari, Yonsei University, Yonsei Proteome Research Center, Centro de Investigación Médica Aplicada, Universidad de Navarra, Universitat Pompeu Fabra, Centre for Genomic Regulation, Universidad Complutense de Madrid, Korea Basic Science Institute, Institute of Biomedical Chemistry, Université du Québec à Montréal, UNSW Sydney, University of Groningen
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: hybrid
Estimated APC paid: $1,700
OpenAlex ID: W659219547