Year: 2024
Type: article
Abstract: \nThis project aims to produce a microfluidic device capable of separating 6 μm and 20 μm diameters particles by inertial sorting. This Lab-on-Chip (LoC) was designed with a trapezoidal cross-section for better fluid control and effective particle manipulation at the microscopic level, as demonstrated by COMSOL simulations. The device was manufactured on a substrate of Polymethyl Methacrylate (PMMA) by femtosecond laser technology and then assembled using an innovative geometry-preserving Isopropyl alcohol-based procedure. The LoC was test with spherical plastic microparticles of two diameters (6 μm and 20 μm) suspended in distilled water. The separation efficiencies were (98.2 ± 1.6) % for 20 μm diameter particles and (70.0 ± 1.8) % for 6 μm diameter particles in good agreement with the simulation results. Finally, after a microfluidic channels’ acetone vapors treatment, the device demonstrated a good ability to separate biological particles (Red Blood Cells) at different concentrations (20%, 30%, 40%, 50%) in a PBS buffer.\n
Authors: Stefania Caragnano, Angeles Ivon Rodriguez Villarreal, Jasmina Casals Terre, Isabella Petruzzellis, Antonio Ancona, Roberto Osellame, Rebeca Martínez Vázquez, Annalisa Volpe
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W6884553714
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