Evolutionary adaptations of doublet microtubules in trypanosomatid parasites.
Type: article
Abstract: The movement and pathogenicity of trypanosomatid species, the causative agents of trypanosomiasis and leishmaniasis, are dependent on a flagellum that contains an axoneme of dynein-bound doublet microtubules (DMTs). In this work, we present cryo-electron microscopy structures of DMTs from two trypanosomatid species, Leishmania tarentolae and Crithidia fasciculata, at resolutions up to 2.7 angstrom. The structures revealed 27 trypanosomatid-specific microtubule inner proteins, a specialized dynein-docking complex, and the presence of paralogous proteins that enable higher-order periodicities or proximal-distal patterning. Leveraging the genetic tractability of trypanosomatid species, we quantified the location and contribution of each structure-identified protein to swimming behavior. Our study shows that proper B-tubule closure is critical for flagellar motility, exemplifying how integrating structural identification with systematic gene deletion can dissect individual protein contributions to flagellar motility.
DOI: 10.48620/87157
Authors: Doran, Matthew H, Niu, Qingwei, Zeng, Jianwei, Beneke, Tom, Smith, James, Ren, Peter, Fochler, Sophia, Coscia, Adrian, Höög, Johanna L, Meleppattu, Shimi, Lishko, Polina V, Wheeler, Richard J, Gluenz, Eva, Zhang, Rui, Brown, Alan
Language: English
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W6892216563
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