Detection of Human Astrocyte Infection With Orthohantaviruses
Year: 2024
Type: article
Abstract: In this report, a technical protocol for the infection of human astrocytes with the orthohantaviruses Dobrava (DOBV) and Puumala (PUUV) and the detection of infected cells is established. After a 12-day incubation period following infection of the astrocytes, the viruses were quantified in the supernatants. The DOBV and PUUV viruses were stained with monoclonal antibodies and visualized with AEC substrate, with the infected cells stained red.
Author: Institute for microbiology and immunology, Faculty of Medicine, University of Ljubljana
Institution: University of Ljubljana
Language: English
Topic: Viral Infections and Vectors
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W6893031109
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