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Conference e-Poster 386; Enhancing Trace DNA profile Recovery in Forensic Casework Samples: Evaluation of Amplicon RX™ Post-PCR Clean-up Kit on GlobalFiler™ PCR Products

Year: 2024

Type: article

Abstract: This study assesses the effectiveness of the Amplicon RX™ Post-PCR Clean-up Kit in improving the sensitivity and accuracy of trace DNA profiling using the GlobalFiler™ PCR Amplification Kit. Comparing 55 samples processed under conventional 30-cycle and modified 29-cycle + Amplicon RX™ protocols, results show a significant RFU increase (from 254 to 454) and enhanced allele recovery with reduced drop-in risk. The method also enables faster, more cost-efficient processing. These findings demonstrate the value of Amplicon RX™ in producing higher-quality DNA profiles from challenging forensic samples.

Source: Zenodo (CERN European Organization for Nuclear Research)

DOI: 10.5281/zenodo.15483156

Authors: Alketbi, Salem K, Naeema S. Aljanahi, Maryam M. Almheiri, Suaad A. Alshehhi, Afra N. Sanqoor, Hussein J. Alghanim

Language: English

Topic: Forensic and Genetic Research

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W6893368521

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