Anti-inflammatory effects and HPLC-PDA quantification of the triterpene saponin-enriched extract of Combretum trifoliatum leaves
Year: 2025
Type: article
Abstract: For the first time, the in vitro anti-inflammatory activity against Nitric oxide (NO) overproduction via Lipopolysaccharide (LPS) injection on RAW264.7 cells as well as the quantitative determination of triterpene saponin-enriched extract from the Combretum trifoliatum leaves (SE-CTL) via High-Performance Liquid Chromatography (HPLC) using Photodiode Array Detector (PDA) technique was investigated. The SE-CTL significantly exhibited the best inhibition of NO (IC50 value of 46.39 ± 3.02 µg/mL). Accordingly, qualitative and quantitative analyses of two main triterpene saponins (1 & 2), were clearly and extensively concentrated by the HPLC-PDA method. Two primary markers (1 & 2) displayed a good linearity (R2 > 0.999) in a relatively wide concentration (ranged from 6.25 to 100 µg/mL). The LOD and LOQ of them were respectively in the range of 2.08–3.12 and 6.94–10.42 µg/mL. Their recoveries were evaluated in the range of 98.56–101.58%. The concentrations of markers in SE-CTL were 0.040 and 0.013% (w/w), respectively.
Source: Figshare
Authors: Son, Nguyen Cong Thai, Vo, Thanh Hoa, Tuoi, Thi Hong, Ngo, Trong Nghia, Dang, Van Son, Huynh, Thi Kim Chi, Bui, Trong Dat, Nguyen, Diep Xuan Ky, Phan, Nhat Minh, Tri, Dinh, Nguyen, Kim Phi Phung, Nguyen, Tan Phat
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W6902214435
FWCI:
Cites:
Cited by:
Related to: