LC-MS/MS analysis and α-glucosidase inhibitory effect of majonoside R2 in Vietnamese ginseng (Panax vietnamensis Ha & Grushv.)
Year: 2024
Type: article
Abstract: Majonoside R2 (MR2), the principal saponin of Vietnamese ginseng (Panax vietnamensis Ha & Grushv.), has the unique structure of ocotillol-type dammarane and showed remarkable biological activities. This paper deals with the new findings in the chemical analysis MR2 by the tandem LC-MS/MS and, especially, its inhibitory activities on α-glucosidase for diabetic management. The developed LC-MS/MS method revealed advantages of high selectivity with specific mass transition from precursor [M + H]+ ion (m/z 784.4) into product ion (m/z 475.1), high sensitivity (calibration range: 5-250 ppb; LOD: 1.5 ppb; LOQ: 5.0 ppb), and high accuracy to support further pharmaceutical analysis of MR2. MR2 and its aglycone ocotillol relatively showed certain inhibitory effects on α-glucosidase in vitro with the IC50 values of 353.05 and 219.64 µg/mL and supported by molecular docking analysis, in which MR2 and ocotillol could play as allosteric inhibitors with high binding affinity (–7.8 and −8.1 kcal/mol) evidenced by bonding interactions.
Source: Figshare
Authors: Dang, Hoang Hiep, Doan, Manh Dung, Dang, Thi Ngan, Nguyen, Thi Hoang Anh, Phuong, Thien Thuong, Van, Tuan Vu, Ngoc, Hieu Nguyen, Nguyen, Huu Tung
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W6902225226
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