Resveratrol mitigates oxidative stress and suppresses HBV replication via modulation of the SIRT1-Nrf2 pathway in liver cells
Year: 2025
Type: article
Abstract: Hepatitis B virus (HBV) infection induces oxidative stress, contributing to liver injury and promoting viral replication through elevated reactive oxygen species (ROS). Resveratrol (RES), a polyphenolic antioxidant, has shown antioxidant and antiviral properties, but its role in modulating the SIRT1-Nrf2 axis under oxidative stress remains unclear. This study investigates RES’s effects on oxidative stress pathways and HBV replication, emphasizing SIRT1-Nrf2 regulation as a potential therapeutic target. LO-2 and HepG2 cells were treated with H₂O₂ (300–1000 μM) with or without RES (50 μM). Cell viability (MTT), SIRT1/pNrf2 expression, ROS/MDA levels, and HBV markers (HBc protein, HBV DNA) were analyzed. Confocal microscopy assessed SIRT1 and pNrf2 localization. RES improved cell viability, upregulated SIRT1, promoted Nrf2 phosphorylation/nuclear translocation, and enhanced antioxidant defenses. It significantly reduced ROS/MDA levels and suppressed HBV replication by decreasing HBc protein and HBV DNA (p < 0.05), indicating an oxidative stress-dependent antiviral effect. RES mitigates oxidative stress and suppresses HBV replication via SIRT1-Nrf2 modulation, offering a novel therapeutic strategy for HBV-related liver damage.
Source: Figshare
Authors: Yang, Gang, Li, Tao, Zhang, Xiong, Wang, Lin, Li, Teng, Zhang, Xin
Language: English
Subfield: Geriatrics and Gerontology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W6902233503
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