Work

Vesicle trafficking analysis

Year: 2025

Type: article

Abstract: SH-SY5Y cells were transduced with pWPI vectors expressing the T30 and CW-T30 isoforms and then, were fixed and stained with antibodies against β-tubulin (SIGMA, T4026), (GFP (ABCAM, 13970), Tau (NUVUSBIO, NB100-822247), LC3 (SIGMA, L7543), EEA1 (BD Biosciences, 610457), Rab11A1 (Invitrogen, 71-5300), CD63 (DSHB, H5C6), LAMP1 (DSHB, H4A3), and with DAPI (4',6-diamidino-2-phenylindole, Merck, 268298). When indicated, cells were treated with LysotrackerRed DND-99 (Invitrogen, L7528) before fixing. Confocal microscopy analysis was performed using Fiji software and vesicles were segmented using CellPose Plug-in.

Source: Figshare

DOI: 10.6084/m9.figshare.28771091

Author: García-Escudero, Vega

Language: English

Topic: Cellular transport and secretion

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W6902253138

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