Optimization of the bio-H2 and lactate production from vinasse and molasses: an experimental approach for the bioenergy development in Brazil
Year: 2025
Type: article
Abstract: This study investigates the efficiency of hydrogen (H2) and lactate (HLa) generation in the co-fermentation of sugarcane vinasse and molasses, aiming to optimize operational strategies through central composite design (CCD) and response surface methodology (RSM). The tests were conducted in 1 L Duran® flasks, with a working volume of 500 mL, under thermophilic conditions (55°C) and pH 7.0. Nine conditions were evaluated by varying co-substrate concentrations (3.9 - 11.1 g COD.L−1) and vinasse proportions (7.6 - 92.4%), identifying the most favourable conditions for H2 and HLa production. High H2 potential (up to 1275 mL.L−1) was observed with 7.5 g COD.L−1 of co-substrates and 7.6% vinasse, mainly attributed to Clostridium. Adding molasses maximized H2 generation, while higher vinasse proportions favoured lactobacilli, increasing HLa concentration and reducing H2. Replicability was ensured, validating tests with high H2 generation and 75% similar results. The tests showed discrepancies in bacterial genus prevalence: the condition with 11.1 g COD.L−1 of co-substrates and 50% vinasse favoured HLa producers (Sporolactobacillus, Lactobacillus, Bacillus), while the condition with only 7.5 g COD.L−1 of co-substrates and 7.6% vinasse favoured H2 producers (Clostridium, Thermoanaerobacterium, Pseudomonas). These findings guide future feeding strategies to optimize bioproduct yield, favouring the generation of target bioproducts and ensuring a holistic view of vinasse and molasses utilization for fermentative H2 biogas production.
Source: Figshare
Authors: Ribeiro, Alexandre Rodrigues, de Freitas, José Arnaldo, Devens, Kauanna Uyara, Sakamoto, Isabel Kimiko, Varesche, Maria Bernadete Amâncio, Silva, Edson Luiz
Language: English
Subfield: Building and Construction
Field: Engineering
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W6902270726
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