Additional file 1 of Recruitment and rejoining of remote double-strand DNA breaks for enhanced and precise chromosome editing
Year: 2025
Type: article
Abstract: Additional file 1: Fig. S1. Homologous donor also promotes dsDNA recombination. Fig. S2. Determination of random rearrangement and pathological rearrangement sites between NHEJ and HRMR in HEK293T cells. Fig. S3. Sanger sequencing chromatograms showed different repair outcome when using NHEJand HRMRat different endogenous loci. Fig. S4. Optimization of the length of homologous arm and insertion of HRMR. Fig. S5. Different donors promote different forms of chromosome rearrangement. Fig. S6. I-SceI enzyme digestion to determine the relative proportion of HR and NHEJ occurrence. Fig. S7. Detailed explanation of the primer insertion method. Fig. S8. Sequence alignment of VEGFA sites edited by HRMR using primer insertion strategy.
Source: Figshare
Authors: Wang, Mingyao, Fu, Pengchong, Chen, Ziheng, Wang, Xiangnan, Ma, Hanhui, Zhang, Xuedi, Gao, Guanjun
Institutions: ShanghaiTech University, Soochow University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W6902272371
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