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Additional file 3 of Cytotoxicity induced by Aeromonas schubertii is orchestrated by a unique set of type III secretion system effectors

Year: 2025

Type: article

Abstract: Additional file 3. List of PCR primers used for plasmid construction via Gibson assembly in this study. Uppercase letters represent the PCR primer regions corresponding to chromosomal DNA of A. schubertii ATCC 43700, while lowercase letters in H1-fw and H2-rv indicate sequences homologous to the linearized pAX2 vector. Italic underlined letters in H1-rv and H2-fw correspond to sequences inserted into the plasmid, including the GSSG linker followed by HiBit-tag, as required.

Source: Figshare

DOI: 10.6084/m9.figshare.29265397

Authors: Michova, Hana, Pliva, Jan, Jirsova, Anezka, Jurnecka, David, Kamanova, Jana

Institution: Czech Academy of Sciences

Language: English

Topic: Aquaculture disease management and microbiota

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W6902283445

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