Additional file 3 of Lumpy skin disease virus suppresses the antiviral response of bovine peripheral blood mononuclear cells that support viral dissemination
Year: 2025
Type: article
Abstract: Additional file 3. GFP expression in MDBK cells and primary fibroblasts infected with GFP recombinant viruses indicates viral replication. (A-H) MDBK cells (A-D) and primary bovine fibroblasts (E-H) were inoculated with recombinant LSDV WT-GFP and LSDV Vac-GFP at MOI=3 for 1 h (T=0). Infected cells were collected and analysed by flow cytometry at the time points indicated. Histograms representing LSDV WT-GFP-infected MDBK cells (A, B) and fibroblasts cells (E, F) show GFP build-up in infected cells at the indicated time points. The percentage of GFP-expressing cells (C, MDBK cells and G, fibroblasts cells) and the mean fluorescence intensity (D, MDBK cells and H, fibroblasts cells) are presented. Values in graphs were expressed as mean± SEM representing three biological replicates (C- D and G- H).
Source: Figshare
Institution: Hebrew University of Jerusalem
Language: English
Subfield: Virology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W6902296073
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