Simultaneous isolation of phyllanthin, hypophyllanthin, and niranthin from crude lignan extract of Phyllanthus amarus leaves by centrifugal partition chromatography (CPC)
Year: 2025
Type: article
Abstract: Centrifugal partition chromatography (CPC) was successfully used for isolation of three lignan compounds such as phyllanthin, hypophyllanthin, and niranthin from Phyllanthus amarus by using a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (2:1:2:1, v/v/v/v) (Arizona R solvent system) in ascending mode. In CPC method, the 250 mL CPC column was filled with the lower stationary phase at a flow rate of 30 mL/min with a maintaining rotation speed at 500 rpm. Then, the upper mobile phase was pumped into the column at a flow rate of 8 mL/min and the rotation speed was set to 1600 rpm. From 0.10 (g) of the crude lignan extract, phyllanthin (1, 25.3 mg, 92.8% purity), hypophyllanthin (2, 7.4 mg, 88.9% purity), and niranthin (3, 14.8 mg, 94.5% purity) were obtained by a single CPC run. The isolated substances were identified by ESI-MS and comparison of retention times with standards.
Source: Figshare
Authors: An, Tran Chieu, Ngoc, Pham Hong, Nhu, Tran Phan Huynh, Nhu, Huynh Luu Gia, Anh, Vo Thi Que, Quynh, Dinh Luu Nhat, Van Trung, Phung
Language: English
Subfield: Analytical Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W6902348559
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