Additional file 13 of Lumpy skin disease virus suppresses the antiviral response of bovine peripheral blood mononuclear cells that support viral dissemination
Year: 2025
Type: article
Abstract: Additional file 13. LSDV GFP viruses disseminated to permissive cells from infected PBMCs by direct contact. PBMCs (A) or primary fibroblasts (B) infected with LSDV-GFP strains were co-cultured with MDBK-Ruby cells constitutively expressing the red fluorescent protein mRuby3. Images were acquired at 4 dpi (A) or at the indicated times (B). White arrowheads depict infected fibroblasts (GFP-positive, Ruby negative), which disseminate at 2 dpi to MDBK-Ruby cells (white stars, double positive for GFP and Ruby). (C) To evaluate the contribution of indirect versus direct LSDV-GFP transmission from infected PBMCs, MDBK cells were either seeded into a transwell inset (0.4 µ membrane pore size) placed into a well containing infected PBMCs (C, left) or directly overlaid on the infected PBMCs (C, right). At 4 dpi, cells were fixed, counterstained with Hoechst (DNA stain) and imaged to reveal GFP-positive cells infected by LSDV-GFP. Scale bar 100 µm (A, B), 500 µm (C).
Source: Figshare
Institution: Hebrew University of Jerusalem
Language: English
Subfield: Virology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W6902351002
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