Toxicological safety, antioxidant effect, and chemoprotection determination of an extract from the babassu mesocarp (Orbignya phalerata Mart.) in preclinical studies
Year: 2025
Type: article
Abstract: Reactive nitrogen species (RNS) and reactive oxygen species (ROS) play a key role in chronic diseases and aging by initiating protein oxidation, impaired cellular membrane damage and functions, and induction of DNA mutations. When endogenous antioxidants are insufficient to counteract oxidant stress, bioactive compounds might serve as physiological therapeutic agents attributed to their ability to neutralize ROS. The aim of this study was to examine the antioxidant, chemoprotective and toxicological aspects of the hydroethanolic extract from Orbignya phalerata Mart. mesocarp using in vitro and in vivo bioassays. The extract presented 9.9 mg/g of total flavonoids, primarily catechin and epicatechin. This extract exhibited no apparent toxicity upon Artemia salina (LC50 > 1000 μg/ml) or Zophobas morio larvae (LD50 > 3000 mg/kg), low hemolysis, genotoxicity, or mutagenicity utilizing Allium cepa cells. Cytotoxicity effects were observed on in L-929 murine fibroblast only at concentrations above 130 μg/ml. This extract effectively neutralized free radicals [DPPH• (EC50 25.3 μg/ml) and ABTS•+ (EC50 52.6 μg/ml)] and reduced H2O2-initiated effects on Saccharomyces cerevisiae. The chemoprotective activity of this extract was also noted in A. salina, L-929 cells, and S. cerevisiae as evidenced from oxidative damage and toxicity tests results. This constitutes the first report describing the toxicological safety profile of a babassu mesocarp extract a rich source of flavonoids (catechin and epicatechin) with antioxidant and chemoprotective activities.
Source: Figshare
Authors: Debia, Nicole, Sousa, Ana Beatriz Santana, de Melo, Beatriz Arêa Cassiano, de Sousa, Athanara Alves, Nobre, Taline Alves, de Oliveira, Victor Alves, de Almeida, José Otávio Carvalho Sena, de Sousa, João Sammy Nery, Arcanjo, Daniel Dias Rufino, de Moraes Alves, Michel Muálem, Ferreira, Paulo Michel Pinheiro, de Castro E Sousa, João Marcelo
Language: English
Subfield: Plant Science
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W6902389573
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