Work

REMEDi4ALL - Standard Operating Procedure - Detection of Drug-Induced-Phospholipidosis

Year: 2024

Type: article

Abstract: This SOP describes the procedure of measuring Drug-Induced-Phospholipidosis staining in VeroE6 cell line in combination with an Operetta HCS system (Revvity) at Fraunhofer ITMP-SP. HCS LipidTOX™ Red Phospholipidosis Detection Reagent (invitrogenTM, #H34351) is a fluorescent dye that is used to stain the cells and to detect induction of phospholipidosis. The dye does not influencing cell growth and is well-retained after aldehyde fixation.

Source: Zenodo (CERN European Organization for Nuclear Research)

DOI: 10.5281/zenodo.13880869

Author: Kuzikov, Maria

Institution: Fraunhofer Institute for Translational Medicine and Pharmacology

Language: English

Topic: Lipid Membrane Structure and Behavior

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funder: European Commission