Work

The synergistic effects of chronic gas condensate pollution and warming on the survival, performance and reproduction of the mussel Brachidontes pharaonis

Year: 2025

Type: article

Abstract: The individual and combined effects of chronic gas condensate pollution and warming of 3.1 °C on the survival, physiological and pathohistological responses of the mussel Brachidontes pharaonis and the mono/poly cyclic aromatic hydrocarbons (M/PAHs) bioconcentration patterns in the mussels' tissues. Clearance rate was measured by incubation of mussels (n=3 from each treatment) in seperate chambers, with the microalgae Tetraselmis tetrathele. Three chambers without mussels were used as control. At the end of the incubation, algal cells were counted under a microscope using a hemocytometer. Clearance rate was standardized to wet weight and shell length and calculated. Respiration rate was measured in seperate chamber of each mussel at the beginning and after one hour using a handheld sensor. Control jars with no mussels were used to account for microbial respiration. Respiration rate (µmol O2 h-1 g WW-1) was calculated per weight and per length. Two-way ANOVA and Tukey post-hoc were used to test the effect of gas condensate concentrations and temperature and their combination on respiration rates and clearance rates using with R v. 4.2.2 using the package “stats” (R Core Team, 2021). Tissue PAHs: triplicate tissue samples were pooled and extracted using a Speed Extractor E-916 (Büchi). Gas chromatography-mass spectrometry (GC/MS, Agilent 6890/5973) was used to identify and quantify the concentrations of each MAH and PAH. Water PAHs: the water was extracted using a solid-phase extraction (SPE) and the samples were analyzed using an Agilent GC/MS (6890/5973) against external selected MAHs standard and 16 PAHs standard Temperature logs: Temperatures in the ambient and warming treatments were monitored and logged hourly during the whole experimental period using temperature loggers (EnvLogger, Electric Blue, Portugal).

Source: Zenodo (CERN European Organization for Nuclear Research)

DOI: 10.5281/zenodo.15342372

Authors: Pressman Tal, Noga, Astrahan, Peleg, Guy-Haim, Tamar

Institutions: Israel Oceanographic and Limnological Research, University of Haifa, Ben-Gurion University of the Negev

Language: English

Topic: Environmental Toxicology and Ecotoxicology

Subfield: Health, Toxicology and Mutagenesis

Field: Environmental Science

Domain: Physical Sciences

SDGs: Life below water, Clean water and sanitation

Open Access status: green

Funder: Ministry of Energy