Deletion of adipocyte FoxO1 activated metabolic pathways and improved systemic metabolic health.
Year: 2024
Type: article
Abstract: (A) Genotyping of FoxO1flox/flox mice and adipoqCreERT2:FoxO1flox/flox mice by PCR and DNA gel imaging. As floxed FoxO1 is located at 149 bp and adipoqCreERT2 at 600 bp, the images indicated that mice #1–2 were of adipoqCreERT2:FoxO1flox/flox and mice #3–4 of FoxO1flox/flox genotype. (B) Western blotting analysis of FoxO1 expression in control and adO1KO mice. Tamoxifen treated FoxO1flox/flox (control, Ctrl) showed normal expression of FoxO1, while tamoxifen-treated adipoqCreERT2:FoxO1flox/flox mice (adO1KO) showed drastic deletion of FoxO1 in white adipose tissue. iBAT, interscapular brown adipose tissues; iWAT inguinal white adipose tissue; gWAT, gonadal white adipose tissue; mWAT, mesenteric white adipose tissue; rWAT, retroperitoneal white adipose tissue. (C–D) adO1KO mice exhibited upregulation of metabolism-regulating genes in gonadal (C) and inguinal (D) adipose tissues. (E–F) KEGG pathway analysis of the upregulated genes in gonadal (E) and inguinal (F) adipose tissues. (G–H) Glucose tolerance test and the area under the curve (n = 6). (I) Insulin tolerance test (n = 8). (J) HOMA-IR indices of the control and adO1KO mice (n = 12). (K–M) Body composition analysis of adO1KO and control mice (n = 8). (N) Measurement of food intake (n = 6). *, p < 0.05; n.s., not significant.
Source: Figshare
Authors: Shi, Limin, Tao, Zhipeng, Zheng, Louise, Yang, Jinying, Hu, Xinran, Scott, Karen, de Kloet, Annette, Krause, Eric, Collins, James, Cheng, Zhiyong
Language: English
Subfield: Physiology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W6920961231
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