Work

Increasing the production of insulin glargine in

Year: 2024

Type: article

Abstract: \nInsulin is the primary drug for managing diabetes mellitus, especially for individuals with type 1 diabetes mellitus. Producing insulin glargine, a long-acting insulin analogue, using Pichia pastoris is a notable advancement in biopharmaceutical manufacturing. This research aims to increase the yield of insulin glargine in Pichia pastoris. The effects of these mediums and vitamins on cell growth and insulin glargine expression levels were evaluated. The findings revealed that the addition of vitamins to the minimal medium (MM) and ½ basal salt medium (BSM) increased insulin glargine production. This study highlights the critical role of vitamins in maximizing the efficiency of insulin glargine production in Pichia pastoris. The addition of vitamins to MM and ½ BSM mediums enhances the production of insulin glargine.\n

Source: Springer Link (Chiba Institute of Technology)

DOI: 10.1051/bioconf/202412706001/pdf

Authors: Dudi Hardianto, Anna Safarrida, Gilang Surya Prayoga, Yufva Pramudia Ananda Zr, Munirah, Efrida Martius, Juwartina Ida Royani, Endah Dwi Hartuti

Institutions: National Research and Innovation Agency, Organisasi Riset Hayati dan Lingkungan, Indonesia Defense University

Language: English

Topic: Fungal and yeast genetics research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W6921841376

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