Year: 2024
Type: article
Abstract: \nWith continuously improving resolution of today’s (super-resolution) microscopes, a major technical limitation of light microscopy based image analysis is linkage error – a visualization error that is measured by the distance between the cellular target to be detected and the fluorescence emitter used for detection. The linkage error of standard labelled antibodies is caused by the size of the antibody and the random distribution of fluorescent emitters on the antibody surface. In this study, we describe a class of staining reagents that effectively reduce the linkage error by more than five-fold when compared to conventional staining techniques. We believe this class of reagents realize an unmet need in cell biological super resolution imaging studies where the precise localization of the target of interest is crucial for the understanding of complex biological phenomena\n
Authors: Vincenzo Manuel Marzullo, Federica Liccardo, Matteo Lo Monte, Giuseppe Palumbo, Marko Lampe, Giuseppe Coppola, Alberto Luini
Institutions: Institute for Experimental Endocrinology and Oncology, National Research Council, University of California, San Francisco, University of Naples Federico II, European Molecular Biology Laboratory, European Molecular Biology Laboratory, Institute of Applied Science and Intelligent Systems
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W6922107209
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