Highly sensitive detection of a neurodegenerative protein biomarker by using the pyro-electrohydrodynamic jet
Year: 2024
Type: article
Abstract: \nA set of protein biomarkers are largely recognized as responsible of neurodegeneration mechanisms and hence as potential targets to be detected in low abundant concentrations in body fluids for performing early diagnosis. As an example, the Tau protein experiences a transition phase from a native disorder conformation into a preaggregation state, which leads to fibrillization processes. Here we show the possibility to detect Tau in urine samples at sub-picogram level, through the concentration effect of the pyro-electrohydrodynamic (p-jet) technique. An immunofluorescence protocol is applied to concentrated p-jet spots able to reduce drastically the diffusion effects in the antibody-antigen reaction. A set of diluted samples were prepared, and the fluorescence signal was detected by a confocal scanner. We achieved an excellent linear response with a significant signal-to-noise ratio down to 0.25 pg/mL. In perspective, the technique could be integrated into a compact device to be used for monitoring the early stage associated to neurodegenerative syndromes in different scenarios such as for example in long-term human space exploration missions.\n
Authors: Concetta Di Natale, Sara Coppola, Veronica Vespini, Volodymyr Tkachenko, Simone Russo, Stefania Carbone, Giuseppina Luciani, Giuseppe Vitiello, Francesca Ferranti, Silvia Mari, Pietro Ferraro, Pier Luca Maffettone, Simonetta Grilli
Language: English
Subfield: Electrical and Electronic Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W6922119025
FWCI:
Cites:
Cited by:
Related to: