Ultra Performance Liquid Chromatography (Uplc): A New Trend in Analysis
Year: 2025
Type: article
Abstract: Ultra Performance Liquid Chromatography (UPLC) is a modern analytical technique widely utilized in analytical chemistry and the pharmaceutical sector. It operates on the principles of column chromatography, which is typically employed to analyze mixtures. UPLC offers innovative advancements in liquid chromatography, allowing for shorter columns, reduced analysis time, and lower solvent usage. By utilizing particles smaller than 2µm, it significantly improves the speed, resolution, and sensitivity of analyses. Ultra Performance Liquid Chromatography (UPLC) leverages advancements in particle chemistry, system optimization, detector technology, and data processing and control to enhance performance. The quality control assessments of different pharmaceutical formulations are being moved from an HPLC system to a UPLC system. The separation in UPLC is carried out at extremely high pressures (up to 100 MPa), yet this does not adversely affect the analytical column or any other parts of the chromatography system. In fact, the efficiency of separation is either preserved or even enhanced by UPLC.
Author: Kiran Gaikwad*1, Vishal Madankar2
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W6930593066