OMIP-113: Characterization of Cytokine Producing T Cells in Swine
Year: 2025
Type: article
Abstract: T cells are essential for preventing diseases and providing long-term protective immunity. The functional capacity of T cells and the quality of their responses to antigens can be measured by the cytokines they produce. We developed a conventional flow cytometry panel utilizing commercially available antibodies to measure antigen specific T cell mediated immune responses in swine. The panel can simultaneously detect Th1 and Th17 cytokines (IFN-γ, TNF and IL-17A) to characterize multifunctional αβ and γδ T cells. We also included CD40L (CD154) to identify cells that are activated upon antigen recall and that may contribute to B-cell help or activate antigen presenting cells. The assay can be applied to study T cell mediated immune responses to vaccines and diseases and can be used with cryopreserved or freshly isolated peripheral blood mononuclear cells.
Authors: Arrigucci, Riccardo, Abby, Patterson, Chloe, Brown, Peter, Dube
Language: English
Topic: T-cell and B-cell Immunology
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W6930645570
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