High-Performance Liquid Chromatographic Technique For Evaluating Solifenacin Succinate Incorporated Into Overactive Bladder Syndrome Treatment
Year: 2024
Type: article
Abstract: The detection of solifenacin succinate was successfully achieved by developing and validating a new, isocratic, rapid, specific, sensitive reversed phase high-performance liquid chromatographic (RP-HPLC) method. A stationary phase of the HiQ Sil C18 (250 x 4.6 mm) column with a temperature of 25oC was used to quantify solifenacin succinate. The mobile phase ACN: Methanol (85.5:14.5 v/v) at a flow rate of 0.7 mL/min was selected to produce the chromatographic separation of solifenacin succinate, and a photodiode array (PDA) detector operating at 221 nm was used for detection. The linear regression analysis results for plotting the calibration curve showed a good linear relation with r2 = 0.999 with the concentration range of 10–60 µg mL−1. 6.15μg mL−1 was the limit of quantitation (LOQ) and 2.02 μg mL−1 was the limit of detection (LOD). The developed technique was determined to comply with ICH guidelines for parameters like specificity, linearity, precision, accuracy, limit of detection, and limit of quantification.
Author: Aakanksha Bhawal, Vaishali Jadhav*, Ashish Jain, Pratiksha Pawar
Language: English
Subfield: Analytical Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W6930767408
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