Work

Comparison of dsRNA and rRNA depleted RNA methods for virome studies in complex plant communities

Year: 2024

Type: article

Abstract: Despite potential roles of natural plant communities as reservoirs of crop pathogens, their viromes are not well understood. Thechoice of robust viral enrichment strategies in complex plant communities is a prerequisite if complex datasets of unknown viralcomposition are to be understood. We use plant communities from arable field margins to compare two methods of viral nucleicacid enrichment and compare three frequently used metagenomics assemblers. Both methods are well established across plantvirome studies; however, they have never been compared in the context of non-synthetic and complex plant communities.

Source: Zenodo (CERN European Organization for Nuclear Research)

DOI: 10.5281/zenodo.16026701

Authors: Niedzicka, Marta, Mc Namara, Louise, Byrne, Stephen

Institution: Teagasc - The Irish Agriculture and Food Development Authority

Language: English

Topic: Plant Virus Research Studies

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: green

Funder: European Commission