Construction of high-yield inositol-1-phosphate synthase recombinant Escherichia coli and optimization of fermentation conditions for myo-inositol production
Year: 2024
Type: article
Abstract: In this study, the recombinant plasmid pETDuet-INO1 was constructed by introducing the expression gene INO1 of inositol-1-phosphate synthase (IPS) (a key enzyme in inositol synthesis pathway) cloned from Saccharomyces cerevisiae SC288 by gene engineering technology into plasmid pETDuet-1. The recombinant strain Escherichia coli BL21/pETDuet-INO1 was constructed by introducing the recombinant plasmid pETDuet- INO1 into E. coli BL21 to achieve efficient expression of IPS in E. coli. The fermentation conditions for myo-inositol production were optimized by single factor experiments and orthogonal experiments. The results showed that the recombinant E. coli BL21/pETDuet-INO1 was successfully constructed, and the optimal fermentation conditions for myo-inositol production were as follows: initial glucose addition 12 g/L, induction temperature 28 ℃, and inoculum 3%. The yield of myo-inositol was (0.81±0.007) g/L after induction 24 h under these conditions, which was 60.39% higher than that before optimization.
Author: YI Minghua, REN Guoping, HU Qiong, QIAN Mengmeng
Institution: Hangzhou Wanxiang Polytechnic
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W6940772003
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